github link
Accession IconGSE96570

Integrated Molecular Analysis of Tamoxifen-Resistant Invasive Lobular Breast Cancer Cells

Organism Icon Homo sapiens
Sample Icon 6 Downloadable Samples
Technology Badge Icon Affymetrix Human Genome U133A Array (hgu133a)

Submitter Supplied Information

Description
Invasive lobular breast cancer (ILC) is an understudied malignancy with distinct clinical, pathological, and molecular features that distinguish it from the more common invasive ductal carcinoma (IDC). Mounting evidence suggests that estrogen receptor-alpha positive (ER+) ILC has a poor response to Tamoxifen (TAM), but the mechanistic drivers of this are undefined. In the current work, we comprehensively characterize the SUM44/LCCTam ILC model system through integrated analysis of gene expression, copy number, and mutation, with the goal of identifying actionable alterations relevant to clinical ILC that can be co-targeted along with ER to improve treatment outcomes. We show that TAM has several distinct effects on the transcriptome of LCCTam cells, that this resistant cell model has acquired copy number alterations and mutations that impinge on MAPK and metabotropic glutamate receptor (GRM/mGluR) signaling networks, and that pharmacological inhibition of either improves or restores the growth-inhibitory actions of endocrine therapy.
PubMed ID
Total Samples
6
Submitter’s Institution

Samples

Show of 6 Total Samples
Filter
Add/Remove
Accession Code
Title
Treatment
Processing Information
Additional Metadata
LCCTam-no4HT, biological rep1
cultured in the absence of 500 nm 4ht for 14d
SUM44+500nM4HT, biological rep3
500 nm 4-hydroxytamoxifen (4ht) for 24h
LCCTam-no4HT, biological rep2
cultured in the absence of 500 nm 4ht for 14d
LCCTam-no4HT, biological rep3
cultured in the absence of 500 nm 4ht for 14d
SUM44+500nM4HT, biological rep1
500 nm 4-hydroxytamoxifen (4ht) for 24h
SUM44+500nM4HT, biological rep2
500 nm 4-hydroxytamoxifen (4ht) for 24h
Loading...