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Accession IconGSE47452

Mouse brain microarray, Chronic HDAC inhibitor treatment

Organism Icon Mus musculus
Sample Icon 36 Downloadable Samples
Technology Badge IconIllumina MouseWG-6 v2.0 expression beadchip

Submitter Supplied Information

Description
Here, we examined mouse brain trancriptional changes 1 hour after the 10th daily i.p. treament with one of the four following treaments: i) vehicle control (45% saline, 45% PEG-400 and 10% DMSO administered at 7.5mL/kg), ii) Cpd-60, 45mg/kg , administered at 7.5mL/kg or iii) SAHA, 25mg/kg, administered at 5mL/kg) or iv) CI-994, 10mg/kg, administered at 5mL/kg. Cpd-60 is a benzamide HDAC inhibitor with selectivity for class I HDAC subtypes HDAC1 and HDAC2; CI-994 is a benzamide inhibitor with selectivity for HDACs1,2 and 3; SAHA is a hydroxamic acid HDAC inhibitor with selectivity for class I HDAC subtypes 1,2, and 3 and the class II HDAC subtype HDAC 6. We examined transcript differences using the Illumnia WG-6 2.0 whole genome expression array and profiled 3 specific brain regions (prefrontal cortex, nucleus accumbens, hippocampus) from each of 36 mice (n=6 mice / treatment group) . For application to array chips, we pooled two biological replicates from like treatment and brain region-groups such that 36 samples were applied in total: 4 treatment groups x 3 brain regions per treament group x 3 pools of two samples each for each treatment/brain region.
PubMed ID
Total Samples
36
Submitter’s Institution

Samples

Show of 36 Total Samples
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Additional Metadata
Vehicle PFC pool mouse 5+6
male
vehicle
SAHA NAc pool mouse 1+2
male
saha 25mg/kg
Vehicle HIP pool mouse 3+4
male
vehicle
Vehicle PFC pool mouse 1+2
male
vehicle
CI-994 NAc pool mouse 1+2
male
ci-994 10mg/kg
Cpd-60 NAc pool mouse 3+4
male
cpd60 45mg/kg
CI-994 HIP pool mouse 3+4
male
ci-994 10mg/kg
SAHA NAc pool mouse 5+6
male
saha 25mg/kg
Cpd-60 HIP pool mouse 1+2
male
cpd60 45mg/kg
Vehicle NAc pool mouse 5+6
male
vehicle
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